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Natural Killer Cells: Methods and Protocols
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(Buch) |
Dieser Artikel gilt, aufgrund seiner Grösse, beim Versand als 5 Artikel!
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| This volume contains collection of Natural Killer Cell methodologies relevant for both
basic and translational research. These methodologies present new developments
in the natural killer (NK) cell field, such as understanding the influence of NK
cells metabolism on its function, identifying complexity of NK cell subsets
through mass cytometry, and determining the emergence of memory NK cells in
murine model of MCMV infection. Methods that study NK cell migration and
cytotoxicity through endpoint analysis or live single cell imaging are also
discussed. Chapters also describe methods pertaining to translational
application of NK cells, such as
ex vivo
expansion of NK cells
on K562 cell lines genetically modified to express either membrane bound IL-15
or membrane bound IL-21, large scale NK cell culture, current techniques for
engineering NK cells to express chimeric antigen receptors or chemokine
receptors using retroviral vectors, electroporation of mRNA, and the
natural phenomenon of trogocytosis. Written in the highly successful
Methods
in Molecular Biology
series format, these chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting edge and thorough,
Natural Killer Cells: Methods and
Protocols
is a valuable resource for researchers who not only want to
understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive
immunotherapy applications.
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Auf Bestellung (Lieferzeit unbekannt)
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