The book begins with an overview of the development of molecular biophysics and a brief survey of  structural, physical, and chemical principles.  Subsequent chapters written by experts present, with examples, the major experimental methods: optical spectroscopy, X-ray and neutron diffraction and scattering, nuclear magnetic resonance, electron paramagnetic resonance, mass spectrometry, and single molecule methods.  The relationship between the biophysical properties of biological macromolecules and their roles as molecular machines is emphasized throughout and illustrated with three examples-DNA helicases, rotary motor ATPases, and myosin.  The concluding chapter discusses future prospects in X-ray and neutron scattering, mass spectrometry, and pharmaceutical development. 

Dr. Norma M. Allewell is Professor of Cell Biology and Molecular Genetics and Affiliate Professor of Chemistry and Biochemistry at the University of Maryland, where she served as Dean of the College of Chemical and Life Sciences for a decade. Her research focuses on protein structure, function and dynamics, and metabolic regulatory mechanisms and diseases.

Dr. Linda Narhi is a Scientific Executive Director in the Product Attribute Science Group at Amgen, where her responsibilities include solution stability assessment of all protein-based therapeutic candidates, and developing andimplementing predictive assays for protein stability to process, storage, and delivery conditions.

Dr. Ivan Rayment is Professor of Biochemistry at the University of Wisconsin-Madison, where he holds the Michael G. Rossmann Professorship in Biochemistry. He has a wide range of interests in structural biology and has made seminal contributions to our understanding of the structural basis of motility, enzyme evolution, cobalamin biosynthesis, and transposition.