This book, Computational Intelligence in Digital Forensics: Forensic Investigation and Applications, is the first volume in the Intelligent Systems Reference Library series. The book presents original research results and innovative applications of computational intelligence in digital forensics. This edited volume contains seventeen chapters and presents the latest state-of-the-art advancement of Computational Intelligence in Digital Forensics; in both theoretical and application papers related to novel discovery in intelligent forensics. The chapters are further organized into three sections: (1) Introduction, (2) Forensic Discovery and Investigation, which discusses the computational intelligence technologies employed in Digital Forensic, and (3) Intelligent Forensic Science Applications, which encompasses the applications of computational intelligence in Digital Forensic, such as human anthropology, human biometrics, human by products, drugs, and electronic devices.