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Herausgeber: 
  • Monalisa Mishra
  • Fundamental approaches to screen abnormalities in Drosophila 
     

    (Buch)
    Dieser Artikel gilt, aufgrund seiner Grösse, beim Versand als 3 Artikel!


    Übersicht

    Auf mobile öffnen
     
    Lieferstatus:   Auf Bestellung (Lieferzeit unbekannt)
    Veröffentlichung:  Januar 2020  
    Genre:  Naturwissensch., Medizin, Technik 
     
    Animal Models / Animal models in research / B / Behavioral Genetics / Behavioral Sciences / Behaviourism, Behavioural theory / bioinformatics / Biological Microscopy / Biology, life sciences / Biomedical and Life Sciences / Cellular biology (cytology) / Computational biology / bioinformatics / Developmental biology / Experimental Organisms / Life Sciences / METABOLISM / Metabolomics / Microscopy / Scientific equipment, experiments & techniques / Zoology & animal sciences
    ISBN:  9781493997558 
    EAN-Code: 
    9781493997558 
    Verlag:  Springer Nature EN 
    Einband:  Gebunden  
    Sprache:  English  
    Serie:  Springer Protocols Handbooks  
    Dimensionen:  H 254 mm / B 178 mm / D  
    Gewicht:  947 gr 
    Seiten:  265 
    Illustration:  VII, 265 p. 116 illus., 111 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen 
    Bewertung: Titel bewerten / Meinung schreiben
    Inhalt:
    This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided.

    The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysisfor various tissues using SEM. In closing, it addresses the analysis of Drosophila's eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.

      
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