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Lieferstatus:	i.d.R. innert 7-14 Tagen versandfertig
Veröffentlichung:	Oktober 2016
Genre:	Naturwissensch., Medizin, Technik
	Applied optics / Biological and Medical Physics, Biophysics / Biological physics / Biophysics / C / Chemistry and Materials Science / Laser / Lasers / Medical physics / Optical physics / Optics, Lasers, Photonics, Optical Devices / Photonics / spectroscopy / Spectroscopy/Spectrometry
ISBN:	9783319354293
EAN-Code:	9783319354293
Verlag:	Springer International Publishing
Einband:	Kartoniert
Sprache:	English
Serie:	#15 - Springer Series on Fluorescence
Dimensionen:	H 235 mm / B 155 mm / D 21 mm
Gewicht:	581 gr
Seiten:	384
Zus. Info:	Paperback
Bewertung:	Titel bewerten / Meinung schreiben

Inhalt:

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.